Custom Peptide Synthesis FAQs
- What synthesis methodologies do CanPeptide use?
- What’s the turn-around time for my peptides?
- What levels of purity are available and what purity is appropriate for my application?
- Do my peptides need N-terminal acetylation and C-terminal amidation?
- Does Canpeptide provide resin-associated peptides?
- How can I know if the biotin is on N-terminal, not somewhere else?
- What purity can be expected from the non-purified and desalted peptides?
- Is there any charge for N-terminus acetylation and C-terminus amidation?
- How are my peptides shipped and what’s the cost?
- What data will be provided along with the product shipping?
- What does CanPeptide do if some problems happen during making my peptides?
- How should I store my peptides? How can I dissolve my peptide when it shows poor solubility?
1. What synthesis methodologies do CanPeptide use?
We use optimized Fmoc chemistry, both solid phase and solution phase methodologies for the peptide production. . Automated or manual peptide synthesis is performed where appropriate.
2. What’s the turn-around time for my peptides?
Generally, the turn-around time for the crude products is 1-2 weeks and 2-3 weeks for the purified lyophilizates. It may vary depending on the peptide length and difficulty.
3. What levels of purity are available and what purity is appropriate for my application?
CanPeptide offers different purity levels of peptides ranging from 70%-95%, or even higher, e.g., >98%, upon request.
Peptides with >70% purity are recommended for screening purposes and polyclonal antibody production. Peptides with >80% purity are also used for antibody production and immunological applications. For ligand binding studies, enzymology, in-vitro bioassay and biological activity studies, we tend to recommend using peptides with a purity of 90% or higher to insure reliability and good experimental reproducibility. For in-vivo studies and structural studies (e.g., NMR and Crystallography), a higher purity of 98% is recommended.
4. Do my peptides need N-terminal acetylation and C-terminal amidation?
These modifications may avoid unnatural charges at the peptide terminals and protect the peptides from degradation resulting from exopeptidases. We suggest having the C-terminal amidation, which make the peptide more closely mimic the charge state in the native protein.
5. Does Canpeptide provide resin-associated peptides?
We can provide you with the peptides attached to the resins through C-terminus, which can be used for library screening. Depending on the applications, peptides can be attached to PS and PEG-based resins, etc.
6. How can I know if the biotin is on N-terminal, not somewhere else?
All other reactive groups are blocked throughout the synthesis and only the free N-terminal is available for biotinylation.
7. What purity can be expected from the non-purified and desalted peptides?
Generally >60%. However, depending on the difficulty of the sequences, this estimation cannot be guaranteed for all cases.
8. Is there any charge for N-terminus acetylation and C-terminus amidation?
No, there is no extra charge for these services.
9. How are my peptides shipped and what’s the cost?
Peptides are shipped via FedEx overnight. Currently, the shipping is at no charge.
10. What data will be provided along with the product shipping?
HPLC chromatogram, Mass Spectra and a data sheet containing the features of the product, such as primary amino acid sequence, modification, quantity packed and purity, etc.
11. What does CanPeptide do if some problems happen during making my peptides?
If due to the synthesis difficulty, the product cannot be delivered on time, we will inform you as soon as possible. However, every endeavor will be made to secure the product quality and the delivery time.
12. How should I store my peptides? How can I dissolve my peptide when it shows poor solubility?
Please take a look at our Storage & Handling guideline or consult our scientist for more information.
If you do not find the answer to your question here, please ask us.